Composite

Part:BBa_K1354000

Designed by: Devora Najjar, Shoshana Sigal   Group: iGEM14_Cooper_Union   (2014-10-03)

UV Promoter with Phage Activator

This is a composite part that used as the input component for our 2-plasmid system. The input BioBrick consists of a promoter gene, in this case it is a SulA UV promoter, with an RBS, the PhiR73 Phage promoter, and a double Terminator. The composite part is flanked by an XhoI site and a EcoRI site. Between the UV promoter and the Phage activator there is a KpnI site that can be used as a way to cut the UV promoter out to swap in other promoters. Before the UV promoter there is a BioBrick prefix. There are start and stop codons flanking the Phage Promoter, along with a scar site. After the double Terminator there is a BioBrick suffix.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None